2 edition of 2-[mu]m plasmid of Saccharomyces cerevisiae found in the catalog.
2-[mu]m plasmid of Saccharomyces cerevisiae
Krisztina Maria Zsebo
Written in English
|Statement||by Krisztina Maria Zsebo.|
|The Physical Object|
|Pagination|| 36 leaves, bound :|
|Number of Pages||36|
The selfish 2 micron plasmid of Saccharomyces cerevisiae utilizes the nuclear motor Kip1p but not Cin8p for its localization and equal segregation, J. Cell Biol. Jayaram, M., Split target specificity of ResT: a design for protein delivery, site selectivity and regulation of enzyme activity, Mol. Microbiol. city proximal to a PAM sequence (2,6). MATERIALS AND METHODS Strains and media The Saccharomyces cerevisiae strain used in the CAN1 mutagenesis analysis of the CRISPR system and the gRNA plasmid/donor DNA transformation in Cas9-ex-pressing cells was BY (MATa his3 D trp1 63 leu2 D0 met15 0 ura3 0), which was a kind gift from Fred Winston.
RPD3 can then repress gene transcription1,2,3,4,5,6. In the yeast Saccharomyces cerevisiae, association of RPD3 with the transcriptional repressors SIN3 and UME6 results in . Abstract. Batch culture experiments of three different strains ofSaccharomyces cerevisiae have been carried out. The first strain was transformed by a plasmid pCYG4, which carries the glutamate dehydrogenase (NADP-GDH, E.C. ) gene conferring an fold increase in activity.
The yeast Saccharomyces cerevisiae has been successfully employed to establish model systems for a number of viruses. Such model systems are powerful tools to study the virus biology and in particular for the identification and characterization of host factors playing a role in the viral infection cycle. Adeno-associated viruses (AAV) are heavily studied due to their use as gene delivery . Protein kinases in the Cot-1/Orb6/Ndr/Warts family are important regulators of cell morphogenesis and proliferation. Cbk1p, a member of this family in Saccharomyces cerevisiae, has previously been shown to be required for normal morphogenesis in vegetatively growing cells and in haploid cells responding to mating pheromone.A mutant of PAG1, a novel gene in S. cerevisiae, displayed defects.
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Conde J, Fink GR. A mutant of Saccharomyces cerevisiae defective for nuclear fusion. Proc Natl Acad Sci U S A. Oct; 73 (10)– [PMC free article] Gerbaud C, Fournier P, Blanc H, Aigle M, Heslot H, Guerineau M. High frequency of yeast transformation by plasmids carrying part or entire 2-micron yeast plasmid.
by: Most strains of the yeast, Saccharomyces cerevisiae, harbor about copies of an autonomously replicating plasmid, the 2-μm gh the plasmid confers no advantage to its host, it is very stable.
Because of its high copy-number and stability, the 2-μm plasmid has been a useful model for studying DNA replication, recombination, regulation of gene expression, and plasmid Cited by: Conde J, Fink GR.
A mutant of Saccharomyces cerevisiae defective for nuclear fusion. Proc Natl Acad Sci U S A. Oct; 73 (10)– [PMC free article] [Google Scholar] Gerbaud C, Fournier P, Blanc H, Aigle M, Heslot H, Guerineau M.
High frequency of yeast transformation by plasmids carrying part or entire 2-micron yeast plasmid. by: The FLP recombinase, encoded by the 2 micron plasmid of Saccharomyces cerevisiae, promotes efficient recombination in vivo and in vitro between its specific target sites (FLP sites).
It was previously determined that FLP interacts with DNA sequences within its target site (B. Andrews, G. Proteau, L.
Beatty, and P. by: The STB-P locus of the 2m plasmid variant found in most laboratory strains of Saccharomyces cerevisiae (Scp1) consists of five tandemly-arrayed repeats (Figure 1A); however, deletion studies and.
Futcher AB, Cox BS. Maintenance of the 2 microns circle plasmid in populations of Saccharomyces cerevisiae. J Bacteriol. May; (2)– [PMC free article] Hartley JL, Donelson JE.
Nucleotide sequence of the yeast plasmid. Nature. Aug 28; ()– Holm C. Clonal lethality caused by the yeast plasmid 2 mu DNA. Cell. The 2 micron plasmid of Saccharomyces cerevisiae is a relatively small multi-copy selfish DNA element that resides in the yeast nucleus at a copy number of 40–60 per haploid cell.
The plasmid is able to persist in host populations with almost chromosome-like stability with the help of a partitioning system and a copy number control system.
The stability of the 2 mu-based yeast plasmid pJDB in Saccharomyces cerevisiae SB(cir0) was investigated in glucose-limited chemostat culture. Plasmid-free cells were detected by loss of (plasmid-encoded) leucine prototrophy and confirmed by colony hybridization.
Hollenberg CP. Mapping of regions on cloned Saccharomyces cerevisiae 2-mum DNA coding for polypeptides synthesized in Escherichia coli minicells. Mol Gen Genet. Jun 1; (1)– Holm C. Clonal lethality caused by the yeast plasmid 2 mu DNA.
Cell. Jun; 29 (2)– Jayaram M, Li YY, Broach JR. Results showed that T‐DNA circles were formed in yeast with precise fusions between the left and right borders. Such T‐DNA circles were stably maintained by the yeast if the replicator from the yeast 2 mu plasmid was present in the T‐DNA.
Integration of T‐DNA in the siae genome was found to occur via homologous recombination. We have developed a system for assaying pyrimidine dimers in the 2 ⇐m DNA plasmid of Saccharomyces cerevisiae, using Micrococcus luteus UV endonuclease to nick dimer-containing plasmid molecules and measuring percentages of nicked and covalently closed circles on agarose gels.
UV-irradiation induced dimers in plasmid DNA, in vivo, at the same rate as in chromosomal DNA. Saccharomyces cerevisiae (/ ˌ s ɛr ə ˈ v ɪ s i. iː /) is a species of has been instrumental in winemaking, baking, and brewing since ancient times.
It is believed to have been originally isolated from the skin of grapes (one can see the yeast as a component of the thin white film on the skins of some dark-colored fruits such as plums; it exists among the waxes of the cuticle). Saccharomyces cerevisiae has been developed as a model eukaryotic organism for a number of reasons, for example.
Saccharomyces cerevisiae is a small single cell with a doubling time of 30 °C of –2 h and importantly can be cultured easily. Consequently, they permit the rapid production and maintenance of multiple strains at low cost. After a 1 h incubation in the growth medium without selection, transformants are obtained on a selective plate medium.
After a short description of the present knowledge on the events affecting the yeast cell as a consequence of the pulsed electric field, a step-by-step protocol is reported for Saccharomyces cerevisiae. Homologous expression of plasmid-encoded Saccharomyces cerevisiae RCE1 under the control of the GAL1 promoter gave a fold increase in endoprotease activity over an uninduced control.
Yeast Rce1p was detected by Western blotting with a yRce1p antibody or with an anti-myc antibody to Rce1p bearing a C-terminal myc-epitope. The copy number of 2 mu DNA-derived plasmids in CIR+ Saccharomyces cerevisiae transformants is determined by its selective marker and is usually much lower than that of the endogenous plasmid.
Only plasmids containing the leu2 allele of pJDB, designated as leu2-d, under selective conditions displayed a higher copy number than did endogenous. Curing Saccharomyces cerevisiae of the 2 micron plasmid by targeted DNA damage. Tsalik EL, Gartenberg MR Yeast.
Jun 30;14(9) PubMed Article Plasmids from Article. ID Plasmid ; Addgene is a nonprofit plasmid repository. We store and distribute high-quality plasmids from your colleagues. Our Repository. Broad-Substrate-Speciﬁcity 2-Oxo Acid Decarboxylase Activity of Saccharomyces cerevisiae Zeynep Vuralhan, 1Marijke A.
Luttik, Siew Leng Tai, Viktor M. Boer,1 Marcos A. Morais,2 Dick Schipper,3 Marinka J. Almering,1 Peter Ko¨tter,4 J. Richard Dickinson,5 Jean-Marc Daran, 1* and Jack T.
Pronk. Saccharomyces boulardii is a tropical species of yeast first isolated from lychee and mangosteen fruit in by French scientist Henri Boulard. Although early reports described distinct taxonomic, metabolic, and genetic properties, S.
boulardii is a strain of S. cerevisiae, sharing >99% genomic relatedness, giving the synonym S. cerevisiae var boulardii.A type strain is Hansen CBS Abstract. transferase, EC ) catalyzes the committed step of phosphatidylethanolamine synthesis via the CDP-etha-nolamine pathway. The gene encoding ethanolamine ki-nase (EKI1) was identified from the Saccharomyces Ge-nome Data Base (locus YDRW) based on its homology to the Saccharomyces cerevisiae CKI1-encoded choline kinase, which also exhibits ethanolamine kinase.
The 2 microns plasmid, Scp1, found in common laboratory strains of Saccharomyces cerevisiae is considered a type-2 plasmid, since the ori, STB, RAF and REP1 loci and intergenic sequences of the.Utilizing human P-glycoprotein (P-gp), we investigated methods to enhance the heterologous expression of ATP-binding cassette transporters in Saccharomyces cerevisiae.
Human multidrug resistance gene MDR1 cDNA was placed in a high-copy 2 mu yeast expression plasmid under the control of the inducible .The 2 micron plasmid of Saccharomyces cerevisiae is maintained by the action of plasmid-encoded gene products that control copy number and promote equipartition of plasmid copies at cell division.